: Ascertain the cycle at which the next by-product of fluorescence with respect to cycle amount is maximal (Rasmussen, 2001). Area fitting to a parabola may very well be used to determine the height placement with sub‐cycle precision. Isopropanol precipitation (Alternate Protocol one) can be used as being a crude https://www.facebook.com/NewTechFind/posts/pfbid02RiZtF2CYQRNXKgPH6trquBJHCScc84k1QCRwYeyZWmLUNxJHv6cRNfFN7v8cEgq9l